Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
3424530 | Virology | 2011 | 4 Pages |
Abstract
The measles virus (MV) glycoproteins hemagglutinin (H) and fusion (F) were recently shown to mediate transduction of resting lymphocytes by lentiviral vectors. MV vaccine strains use CD46 or signaling lymphocyte activation molecule (SLAM) as receptor for cell entry. A panel of H protein mutants derived from vaccine strain or wild-type MVs that lost or gained CD46 or SLAM receptor usage were investigated for their ability to mediate gene transfer into unstimulated T lymphocytes. The results demonstrate that CD46 is sufficient for efficient vector particle association with unstimulated lymphocytes. For stable gene transfer into these cells, however, both MV receptors were found to be essential.
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Authors
Qi Zhou, Irene C. Schneider, Manuela Gallet, Sabrina Kneissl, Christian J. Buchholz,