| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 3424676 | Virology | 2012 | 9 Pages |
Puumala (PUUV) and Hantaan (HTNV) viruses are hantaviruses within the family Bunyaviridae and associated with Hemorrhagic Fever with Renal Syndrome (HFRS) in humans. Little is known about how these viruses interact with host cells, though pathogenic hantaviruses interact with αvβ3 integrin. To study host cell interactions and rapidly test the ability of antibodies to prevent infection, we produced HTNV and PUUV pseudovirions on a vesicular stomatitis virus (VSV) core. Similar to replication-competent hantaviruses, infection was low-pH-dependent. Despite broad cell tropism, several human T cell lines were poorly permissive to hantavirus pseudovirions, compared to VSV, indicating a relative block to infection at the level of entry. Stable expression of αvβ3 integrin in SupT1 cells did not restore infectivity. Finally, the pseudovirion system provided a rapid, quantitative, and specific method to screen for neutralizing antibodies in immune sera.
► We produced infectious HTNV and PUUV pseudovirions on a vesicular stomatitis core. ► Both pseudovirions infect specifically and in a pH-dependent manner. ► Both pseudovirions exhibit broad cell tropism but infect hematopoetic cells poorly. ► αvβ3 integrin expression in SupT1 cells does not restore pseudovirion infectivity. ► Pseudovirions can detect neutralizing antibodies in sera from vaccinated animals.
