Article ID Journal Published Year Pages File Type
3426105 Virology 2007 11 Pages PDF
Abstract

We studied the interaction of RV with human peripheral blood mononuclear cells (PBMC) from adult volunteers. After exposure of PBMC to rhesus RV (RRV), T and B lymphocytes, NK cells, monocytes, and myeloid and plasmacytoid dendritic cells expressed RV non-structural proteins, at variable levels. Expression of these RV proteins was abolished if infection was done in the presence of anti-VP7 neutralizing antibodies or 10% autologous serum. Supernatants of RRV exposed PBMC contained TNF-α, IL-6, IFN-α, IFN-γ, IL-2 and IL-10. Plasmacytoid DC were found to be the main source of IFN-α production, and in their absence the production of IFN-γ and the frequency of RV specific T cells that secrete IFN-γ diminished. Finally, we could not detect RV-antigen associated with the PBMC or expression of RV non-structural proteins in PBMC of acutely RV-infected children. Thus, although PBMC are susceptible to the initial steps of RV infection, most PBMC of children with RV-gastroenteritis are not infected.

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Life Sciences Immunology and Microbiology Virology
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