Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
3427554 | Virology | 2006 | 6 Pages |
Abstract
Substitutions were engineered individually and in combinations at the fusion loop, receptor-binding domain and a stem-helix structure of the envelope protein of a West Nile virus strain, NY99, and their effects on mouse virulence and presentation of epitopes recognized by monoclonal antibodies (MAbs) were assessed. A single substitution within the fusion loop (L107F) attenuated mouse neuroinvasiveness of NY99. No substitutions attenuated NY99 neurovirulence. The L107F mutation also abolished binding of a non-neutralizing MAb, 3D9, whose epitope had not been previously identified. MAb 3D9 was subsequently shown to be broadly cross-reactive with other flaviviruses, consistent with binding near the highly conserved fusion loop.
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Authors
Shuliu Zhang, Li Li, Sara E. Woodson, Claire Y.-H. Huang, Richard M. Kinney, Alan D.T. Barrett, David W.C. Beasley,