| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 3428429 | Virus Research | 2014 | 7 Pages |
•The impact of high throughput sequencing in plant virus characterization is summarized.•The potential of high throughput sequencing in plant virus diagnostic is highlighted.•The limitations that need to be addressed to achieve this potential are discussed.•Wide adoption of HTS in plant virus diagnostic will require time and specific efforts.
The ability to provide a fast, inexpensive and reliable diagnostic for any given viral infection is a key parameter in efforts to fight and control these ubiquitous pathogens. The recent developments of high-throughput sequencing (also called Next Generation Sequencing – NGS) technologies and bioinformatics have drastically changed the research on viral pathogens. It is now raising a growing interest for virus diagnostics. This review provides a snapshot vision on the current use and impact of high throughput sequencing approaches in plant virus characterization. More specifically, this review highlights the potential of these new technologies and their interplay with current protocols in the future of molecular diagnostic of plant viruses. The current limitations that will need to be addressed for a wider adoption of high-throughput sequencing in plant virus diagnostics are thoroughly discussed.
