Article ID Journal Published Year Pages File Type
3428459 Virus Research 2014 4 Pages PDF
Abstract

•Four tobacco rattle virus isolates were identified from tobacco bait seedlings planted in soil samples from Polish potato fields.•RNA1 of the tested isolates showed significant similarity to those of the isolates Ho and AL recently found in Germany.•RNA2 of the isolates Deb57 and Slu24 from northern Poland are in a cluster with the isolates PSG and PLB found in the Netherlands.•RNA2 of the isolates 11r21 and Mlo7 from central Poland are in a distinct group with the unique isolate SYM found in England.

Four tobacco rattle virus (TRV) isolates were identified from tobacco bait seedlings planted in soil samples from Polish potato fields. Sequence analysis of the genomic RNA1 of the isolates revealed significant similarity to the isolates Ho and AL recently found in Germany. Multiple sequence alignments of the genomic RNA2 indicated that the two isolates from northern Poland (Deb57 and Slu24) are in a cluster with the isolates PSG and PLB found in the Netherlands. The remaining two isolates, from central Poland (11r21 and Mlo7), are in a distinct group with the unique isolate SYM found in England. The RNA2 sequences of the studied isolates range from 1998 nt to 2739 nt in length, and all carry deletions of the 2b and/or 2c genes. The isolate Mlo7 has an atypical RNA2 structure, having its cp gene located in its central region.

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