Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
3428468 | Virus Research | 2014 | 5 Pages |
Abstract
•A full-length infectious clone (pMEV) of mink enteritis virus was constructed.•pMEV was infectious when transfected into F81 cells.•rMEV retained the biological properties of MEV-L.
Construction and characterization of a full-length infectious clone (pMEV) of mink enteritis virus are described. Feline kidney cells (F81) were transfected with pMEV containing an engineered BamHI site that served as a genetic marker. The rescued virus was indistinguishable from its parental virus. The availability of a MEV infectious clone will facilitate studies of viral replication and pathogenicity and will permit the elucidation of determinants of the host range of the parvovirus.
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Authors
Daoli Yuan, Jigui Wang, Zhili Li, Yaping Mao, Jia-zeng Sun, Ji Xi, Shuang Wang, Qiang Hou, Bao Yi, Weiquan Liu,