Article ID Journal Published Year Pages File Type
3428468 Virus Research 2014 5 Pages PDF
Abstract

•A full-length infectious clone (pMEV) of mink enteritis virus was constructed.•pMEV was infectious when transfected into F81 cells.•rMEV retained the biological properties of MEV-L.

Construction and characterization of a full-length infectious clone (pMEV) of mink enteritis virus are described. Feline kidney cells (F81) were transfected with pMEV containing an engineered BamHI site that served as a genetic marker. The rescued virus was indistinguishable from its parental virus. The availability of a MEV infectious clone will facilitate studies of viral replication and pathogenicity and will permit the elucidation of determinants of the host range of the parvovirus.

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Life Sciences Immunology and Microbiology Virology
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