Article ID Journal Published Year Pages File Type
3428490 Virus Research 2014 4 Pages PDF
Abstract

•A recombinant baculovirus expressing EAV gP5 was constructed.•The expression of EAV gp5 protein showed cytotoxicity effect in insect cells.•PRRSV gP5 protein was associated with a strong cytotoxicity in culture cells.•Several observed features in insect cultures were correlated with the induction of apoptosis.•We identify a viral gene product that could be a key-factor in apoptosis induction.

Equine Arteritis Virus (EAV) has been shown to induce apoptosis in vitro but the induction of this mechanism has not been previously associated with any viral gene product. In this work, we found a cytotoxicity effect of the EAV gP5 protein on baculovirus-insect cells and a low yield of protein recovery. Besides, different morphological features by electron transmission microscopy, DNA fragmentation in agarose gel, TUNEL analysis and caspase 3 activity were found. All these findings indicate that the EAV gP5 protein induces apoptosis in insect cells.

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Life Sciences Immunology and Microbiology Virology
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