Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
3428767 | Virus Research | 2012 | 4 Pages |
Abstract
The Varicella-zoster virus (VZV) ORF54 gene was characterized using a guinea pig antiserum prepared to a GST-pORF54 fusion protein. A protein of the predicted size, 87 kDa, was detected in VZV-infected MeWo cells but not in mock-infected cells. Sucrose density gradient fractionation of pORF54 expressed in a recombinant baculovirus system resulted in samples containing enriched amounts of pORF54. Electron microscopic analysis suggested that the ORF54 gene encodes a protein that assembles into ring-like portal structures similar to those observed for numerous bacteriophages and other herpesviruses.
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Authors
Alexander J. Howard, Debra M. Sherman, Melissa A. Visalli, Denise M. Burnside, Robert J. Visalli,