Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
3428987 | Virus Research | 2011 | 4 Pages |
Abstract
In the present study, seven new defective interfering (DI) RNA species of porcine reproductive and respiratory syndrome virus (PRRSV) were identified. RT-PCR, Northern blot and sequence analyses indicated that these DI RNA specie have deletions of 8513–9176 nucleotides located between Nsp1/Nsp2 and Nsp10. Compared with the previous DI RNAs of PRRSV reported, they have three distinct characteristics: much smaller deletion sizes; different nucleotide repeats (2–12 nt) used in the junction sites and in-frame deletions. The results further suggested that the similarity-assisted RNA recombination may be the main cause of generation of DI RNAs in PRRSV and probably in other arteriviruses.
Related Topics
Life Sciences
Immunology and Microbiology
Virology
Authors
Chao-Ting Xiao, Zhong-Hua Liu, Xing-Long Yu, Meng Ge, Run-Cheng Li, Bo-Ren Xiao, Hong-Rui Zhou,