| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 3429008 | Virus Research | 2011 | 7 Pages |
To investigate the potential of cross-species transmission of non-human primate HBV to humans, severe combined immunodeficiency mice transgenic for urokinase-type plasminogen activator, in which the mouse liver has been engrafted with human hepatocytes, were inoculated with non-human primate HBV. HBV-DNA positive serum samples from a gibbon or orangutan were inoculated into 6 chimeric mice. HBV-DNA, hepatitis B surface antigen (HBsAg), and HB core-related antigen in sera and HBV cccDNA in liver were detectable in 2 of 3 mice each from the gibbon and orangutan. Likewise, applying immunofluorescence HBV core protein was only found in human hepatocytes expressing human albumin. The HBV sequences from mouse sera were identical to those from orangutan and gibbon sera determined prior to inoculation. In conclusion, human hepatocytes have been infected with gibbon/orangutan HBV.
► uPA/SCID mice with human hepatocytes as an infection model for human hepatitises. ► Cross-species transmission potential of gibbon and orangutan HBV in mouse model. ► HBV markers are found in sera and liver tissues of mice. ► HBV sequences from mouse sera were identical to orangutan and gibbon HBV. ► Human hepatocytes have been infected with gibbon/orangutan HBV.
