Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
3429114 | Virus Research | 2011 | 5 Pages |
Previous work showed that distinct amino acid motifs are encoded by the Rep, Cap and ORF3 genes of two subgroups of porcine circoviruses (PCV), PCV2a and PCV2b. At a specific location of the gene, a certain amino acid residue or sequence is preferred. Specifically, two amino acid domains located in the capsid protein, designated motif-1 (six residues located at positions 86–91) and motif-2 (four residues at positions 190-191-206-210), have been identified to associate with either PCV2a (motif-1a:TNKISI and motif-2a:SRKD) or PCV2b (motif-1b:SNPRSV and motif-2b:AGIE) preferentially. In this study, the protein distribution pattern of a PCV2a isolate and a PCV2b isolate was examined. Each virus exhibited a different viral protein pattern during replication in porcine kidney cells and the viral protein distribution pattern was associated with amino acid motif-2 but not motif-1. The results also showed that a more robust accumulation of viral proteins in the nucleus was associated with motif-2b than with motif-2a. In addition, viruses containing motif-2b replicated better than viruses containing motif-2a in porcine kidney cells.