| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 3447599 | Archives of Medical Research | 2006 | 6 Pages |
BackgroundThe aim of this study was to investigate the effect of static magnetic field (SMF) exposure on testicular function, antioxidant status and DNA oxidation in rats.MethodsMale adult rats were exposed to SMF (128 mT; 1 h/day for 30 days). After sacrifice, the epididymal sperm number was counted. Testosterone concentration in plasma and testis was measured by radioimmunoassay. MDA level and GPx, CAT and SOD activities were used as markers of oxidative stress in testis. The 8-oxo-dG level is measured by the HPLC-EC system.ResultsSubchronic exposure to SMF has no effect on epididymal sperm count, spermatozoa motility and genital organ weight. In contrast, SMF induces a decrease of testicular and plasmatic testosterone levels, respectively (1.48 ± 0.56 vs. 4.66 ± 0.51 ng/g, p<0.05; 0.97 ± 0.16 vs. 1.64 ± 0.18 ng/mL, p<0.05). Exposed rats displayed an increase of malondialdehyde (2.01 ± 0.03 vs. 1.47 ± 0.06 μmol/g protein, p<0.05), metallothioneins (1.04 ± 0.22 vs. 0.37 ± 0.06 μg/g, p<0.05) and 8-oxo-dG concentrations (3.38 ± 0.30 vs. 2.36 ± 0.28 8-oxo-dG/106 bases, p<0.05) in the testis. In the gonad, SMF decreases the CAT (14.33 ± 1.16 vs. 21.67 ± 2.05 U/mg protein, p<0.05), GPx (177.40 ± 5.97 vs. 237.20 ± 15.65 U/mg protein, p<0.05) and mitochondrial Mn–SOD (2.95 ± 0.10 vs. 3.53 ± 0.29 U/mg protein, p<0.05) activities. However, cytosolic CuZn–SOD activity is unaffected.ConclusionsSubchronic exposure to SMF failed to alter spermatogenesis in rat testis. In contrast, the same treatment decreased testosterone levels and induced DNA oxidation.
