FAS-670 A/G and FAS-1377 G/A polymorphism in cell death pathway gene FAS and human male infertility

ObjectiveTo study the role and association of functional variations present in FAS gene with idiopathic male infertility.MethodsThe case-control study comprised of two groups: 160 idiopathic infertile nonobstructive azoospermia patients and 200 fertile healthy control men. Genotyping for single-nucleotide polymorphism of FAS-670 A/G (rs1800682) and FAS-1377 G/A (rs2234767) was done by PCR-RFLP method. DNA sequencing was used to ascertain PCR-RFLP results. For FAS-670 A/G and FAS-1377 G/A functional polymorphism, allele and genotype distribution were evaluated using Chi-square test.ResultsAllele and genotype distribution did not differ significantly between patients and controls for FAS-670 A/G and FAS-1377 G/A.ConclusionsHuman male infertility is a complex disorder and thus other genetic or environmental factors may be contributing to the complex etiology.