Exploiting the role of various types of ion-channels against chemically induced inflammatory bowel disease in male Wistar rats

ObjectiveTo evaluate the role of nifedipine (L-type of calcium channel blocker), glibenclamide [cystic fibrosis transmembrane conductance regulator (CFTR) channel blocker], niflumic acid (chloride channels blocker) and 2-aminoethyldiphenyl borate (calcium release-activated channels channel blocker) against chemically induced inflammatory bowel disease in male Wistar rats.MethodsInflammatory bowel disease was induced by the administration of 2,4-dinitrobenzene sulfonic acid intracolonically. Animals were divided into 7 groups by keeping 6 animals in each group. Group one and two were treated as normal control and disease control, respectively and seven pretreated with sulfasalazine and remaining groups were treated with test drugs. Animals were pretreated for 5 days before administration of 2,4-dinitrobenzene sulfonic acid. Various parameters were recorded during and after treatment period like disease activity index (body weight, food intake, diarrhea score, occult blood score), biomarkers study in peritoneal macrophages and histopathology of colon.ResultsGlibenclamide had offered significant protective effect on disease activity index, biochemical parameters confirmed by histopathological examination. 2-Aminoethyldiphenyl borate and niflumic acid had also shown insignificant protection in few parameters.ConclusionsProtection observed by pretreatment of glibenclamide is may be of its blocking effect of CFTR channels of the mast cells, which regulate chloride influx and efflux phenomenon.