Determination of the total phenols, flavonoids and antimicrobial activity of the crude extracts from locally grown neem stems

ObjectiveTo prepare the crude extracts from the stems of neem with maceration extractor and determine their total phenolics, flavonoids contents and antimicrobial activity by established methods.MethodsThe dry stem samples were used to prepare different crude extracts using maceration method. The total phenolics and total flavonoids contents were determined through Folin-Ciocalteu's reagent and AlCl3 methods. The antimicrobial activity of the crude extracts from the local neem was determined by disc diffusion method against one Gram-positive Staphylococcus aureus and three Grams-negative Escherichia coli, Pseudomonas aeruginosa and vulgaris pathogenic bacterial strains. The standard amoxicillin was used as a positive control for the antimicrobial study.ResultsThe results of the total phenolics content of the crude extracts from different stems ranged from 20.80 mg/100 g to 107.29 mg/100 g of the powder crude extracts. The total flavonoids content of different crude extracts from powder stems of neem ranged from 136.50 mg/100 g to 484.50 mg/100 g of the powder samples. The antimicrobial activity of the crude extracts from different stems of neem at different concentrations against four bacterial strains didn't give any activity.ConclusionsThanks to the contents of the total phenolics and flavonoids, the crude extracts could be used as medicines for different aliments.