Survey for the presence of Neospora caninum in frozen bull's semen samples by PCR assay

ObjectiveTo evaluate the occurrence of Neospora caninum (N. caninum) DNA using polymerase chain reaction (PCR) technique in frozen semen samples of Iranian bulls that were used for artificial insemination.MethodsIn this study, 57 frozen bull's semen samples were collected randomly from artificial insemination centers and genomic DNA was extracted. For detection of pNC-5 gene of N. caninum by oligonucleotide primers were amplified using PCR technique and 1% agarose gel electrophoresis used for visualization of amplified PCR products.ResultsThe results of this study present 6 of 57 (10.53%) as positive samples. This show high presence of N. caninum infection and display importance of frozen semen samples of bulls, which were used for artificial insemination, in the spread of bovine neosporosis in Iran. These results present PCR as suitable technique for fast detection of this protozoan parasite in semen specimens.ConclusionsAccording to these results control and eradication programs, for example prevent vaccinations, as reduction of economic losses caused by this protozoan infection in Iranian cattle seems to be necessary.