Antigenicity of envelop and non-structural proteins of dengue serotypes and their potentiality to elicit specific antibody

ObjectiveTo find out the antigenic nature of envelop (E) and non-structural (NS) proteins and their ability to induce specific antibodies, and to investigate specific antibody produced by specific dengue virus (DENV) serotypes.MethodsAmino acid sequences of E and NS proteins of dengue serotypes were analysed by using VaxiJen antigen predicition server. The transmembrane of topology analyses were conducted by using transmembrane prediction using hidden markov models. The Hex dock server was used for docking.ResultsThe antigenicity score and exomembrane potentiality of E and NS proteins were calculated. All those proteins were antigenic; these antigens were made to interact with antibodies such as immunoglobulin A, immunoglobulin G and immunoglobulin M. Higher energy values of immunoglobulin M were found in DENV-1 and DENV-2, and more energy values were found in immunoglobulin G of DENV-3, DENV-4, NS-1, NS-3 and NS-5.ConclusionsIn the present study, DENV-1 and DENV-2 are positive to immunoglobulin M and involved in the primary infection. DENV 3, DENV 4 and all the NS proteins (NS-1, NS-3, NS-5) which elicit immunoglobulin G are involved in the secondary infection.