Evaluation of the anticancer potentials of Origanum marjorana on fibrosarcoma (HT-1080) cell line

ObjectiveTo evaluate the potential anticancer activity of Origanum majorana (marjoram) (O. marjorana) on the fibrosarcoma cancer cell line HT-1080 through various in-vitro assays.MethodsCrude extracts were prepared from O. marjorana using ethanol, methanol and water as the solvents following standard protocols. Cytotoxicity was assessed using MTT assay, trypan blue dye exclusion, AO/EB staining and fluorescence microscopical analysis and DNA fragmentation analysis.ResultsEthanol extract has shown significant cytotoxicity (P<0.001) to fibrosarcoma and least toxicity to normal human lymphocytes when compared to the controls.ConclusionsThis study shows that O. marjorana ethanol extract has anticancer potentials and can be explored further for active component isolation, identification and characterization.