Antioxidant and anti-inflammatory activity of Polygonatum sibiricum rhizome extracts

ObjectiveTo investigate the antioxidant and anti-inflammatory activities of distilled water extract of fresh Polygonatum sibiricum (P. sibiricum) rhizome.MethodsThe extracts were tested for antioxidant activity by using DPPH (1,1-diphenyl-2-picryl-hydrazyl), and hydroxyl (OH•) radicals scavenging activity. The level of intracellular reactive oxygen species (ROS) was determined in H2O2 treated chang liver cells. Anti-inflammatory activity was evaluated by the inhibition of nitric oxide (NO), inducible nitric oxide synthase (iNOS), as well as tumor necrosis factor-alpha (TNF-α) protein expression in a lipopolysaccharide stimulated Raw 264.7 murine macrophages cell line.ResultsP. sibiricum water extracts scavenged DPPH, OH• radicals and decreased the level ROS. The extracts reduced NO reduction and inhibited the expression of iNOS and TNF-α proteins.ConclusionsThe findings indicate that water extracts of P. sibiricum could be considered as natural antioxidants and anti-inflammatory agents for food and drug industries.