Evaluation of Polyherbal formulation for Diuretic activity in albino rats

ObjectiveTo investigate the diuretic potential of Polyherbal formulations prepared from the seeds of Vitis venifera, Duranta repens and Centratherum anthelminticum in Albino rats.MethodsDifferent concentrations of Polyherbal formulations (100, 200 and 400 mg/kg), furosemide (10 mg/kg) and vehicle were orally administered to rat (n = 6 animals per group) and their urine output was collected after 24h. The Urinary output, Osmolarity, pH, Na+, K+ and Cl− concentrations of urine were estimated.ResultsThe Polyherbal formulations produced significant increase in Na+, K+, Cl− excretion, caused alkalinization of urine, showed strong Diuretic index, saluretic index and Natruretic index. All the concentrations of Polyherbal formulation showed dose-dependent relationship when compared to control animals.ConclusionsThese finding strongly suggests that the Polyherbal formulations have a good diuretic activity on rats in the above experimental model.