In Vitro Free Radical Scavenging Activity of Gallic Acid Isolated From Caesalpinia Decapetala Wood

ObjectiveAntioxidant capacities and phenolic contents of Gallic Acid isolated from plant Caesalpinia decapetala were investigated. Caesalpinia decapetala is a plant used to treat burns, biliousness and stomach disorders.MethodsThe antioxidant activity was estimated by the ABTS and DPPH methods. The total phenolic was measured using Folin-Ciocalteu assay. Identification of isolated Gallic Acid was also performed by reverse-phase high-performance liquid chromatography (RP-HPLC) using Cosmosil C18 column (150mm × 4.6mm, 5μm particle). The mobile phase was a mixture of ethyl acetate: ethanol: water (1:5:4, v/v/v) delivered at a flow rate of 1.0 mL min-1.ResultsThe total phenolic content was found to be 4.31% (w/w). Isolation of Gallic Acid with optimum yield was performed using a mixture of solvents (Ethanol: Water 65:35). Isolated Gallic Acid showed significant in vitro free radical scavenging activity in both model; but in ABTS assay significant % inhibition of free radical was observed as compared to DPPH assay.ConclusionsResearch concluded that Caesalpinia decapetala extract can be used as potent antioxidant which can play vital role against the diseases like neurodegenerative disorders, inflammation, viral infections and gastric ulcer.