Detection and quantification of quercetin in roots, leaves and flowers of Clerodendrum infortunatum L.

ObjectiveTo detect and quantify the concentrations of Quercetin in the root, leaves and flower test sample of Clerodendrum infortunatum L.MethodIn the present study High Performance Thin Layer Chromatography has been developed for detection and quantification of Quercetin in Clerodendrum infortunatum L. Increasing serial dilutions of reference standard Quercetin (20 to 100 μg/ml) were scanned at 366 nm to detect and quantify the concentrations of Quercetin in the test sample.ResultThe estimated values obtained from the same were 0.05mg/g, 0.111mg/g and 0.199mg/g of Quercetin in powdered root, leaves and flower sample respectively.ConclusionThe method provided a rapid and easy approach for detection and the quantitation of the bio-marker Quercetin. In the present study we established the HPTLC profile for the vegetative and reproductive parts of Clerodendrum infortunatum L. to detect and quantify the Quercetin.