Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
3454998 | Asian Pacific Journal of Tropical Disease | 2014 | 4 Pages |
ObjectiveTo examine Beauveria bassiana (B. bassiana) fungus bioassay against the larval stages of Anopheles stephensi in Iran.MethodsThe fungal suspension by the concentrations of 1×109, 5×108, 108, 5×107 and 1×107 conidia per milliliter have been prepared in different volumes (2, 4 and 6 mL) and each concentration were added to containers containing 25 Anopheles larva instars 1 and 2. The mortality of the dead larvae with abnormal symptoms was recorded as a result of the fungal infection after 24, 48 and 72 h.ResultsComparison between the mean mortality rate of Anopheles stephensi larva at different concentrations of B. bassiana strain Iran 429C at 2, 4 and 6 mL showed that there was no significant relation of the mean mortality rate of larvae at concentrations of 1×109 and 5×108, and after 48 h resulted in 100% mortality rate of the larvae populations. In addition, there is no significant differences in the amounts of lethal times (LT) (LT50 and LT90) as LT90 values calculated at a concentration of 5×108 and in volumes 2, 4 and 6 mL were 1.46, 1.36 and 1.08 d, respectively.ConclusionsB. bassiana strain Iran 429C in 2 mL of 5×108 concentration or the concentration of a 1×109 mL per 100 mL of water is recommended as the optimal concentration for the control of Anopheles larvae. The development of suitable formulations of entomopathogenic fungi may be a promising prospect in the mosquito control programs.