Detection of influenza A virus RNA in birds by optimized Real—Time PCR system

ObjectiveTo evaluate the use of Real-Time PCR system based on specific amplification of matrix protein gene fragment for influenza A virus RNA detection in cloacal swabs from wild birds.MethodsSensitivity, specificity and reproducibility of analysis results were identified. Study of cloacal swabs from wild birds for influenza A virus presence was performed.ResultsReproducibility of low concentrations of virus detection in samples by Real-Time PCR was significantly higher than that of detection based on cytopathic effect of viruses grown on MDCK cell culture.ConclusionsReal-Time PCR system for influenza A virus RNA detection is developed and applied for virus surveillance study.