| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 3479329 | Journal of the Formosan Medical Association | 2012 | 5 Pages |
Background/PurposeAccurate and timely diagnosis is vital for the clinical management of influenza. A 2009 pandemic influenza A (H1N1)-specific enzyme-linked immunosorbent assay (ELISA) was tested with selected clinical samples.MethodsA selection of 90 throat swab samples with various viral loads of 2009 pandemic influenza A (H1N1) were tested.ResultsUsing the reverse transcriptase polymerase chain reaction as a gold standard, the overall sensitivity (0.57) was higher than that of the QuickVue Influenza A+B Test (0.43). The specificity of the ELISA was 1.0 using the selected sample set. The positive and negative predictive values were 1 and 0.4, respectively.ConclusionThe ELISA is an easy to perform, highly specific, and fairly sensitive diagnostic tool for the 2009 pandemic influenza A (H1N1) virus infections. A strong correlation was found between viral load and specificity.
