Progress of safflower (Carthamus tinctorius L.) regeneration through tissue culture

BackgroundSafflower regeneration through tissue culture has long been limited to low frequency and lack of an efficient protocol that suitable for most safflower cultivars. In past decades, researches had been carried out to investigate safflower regeneration through tissue culture and great progress had been made.ObjectiveTo investigate factors that affect safflower regeneration through tissue culture principally.MethodsThis article summarized available literatures about advancements in safflower regeneration, especially discussed factors affecting safflower tissue culture in detail.ResultsSafflower regeneration was fairly hard than other congeneric plants, such as chrysanthemum. The genotype, seedling age, type of explants, medium components, plant growth regulators and other additives all had specific influences on safflower tissue culture. More deepgoing researches need to be undertaken to establish an effective safflower regeneration system.