Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
3483964 | Journal of Nanjing Medical University | 2009 | 6 Pages |
ObjectiveTo investigate the expression of herg1 gene in tumor tissues from gastric carcinomas and gastric carcinoma cell lines, and study the relationship between HERG K+ channel expressions and tumor cell proliferation and apoptosis.MethodsRT-PCR and PCR assays were used to detect the expression of herg1 gene in 64 gastric carcinomas and the gastric cancer cell line SGC-7901. Blocking the HERG K+ channels was used to evaluate their effects on tumor cell proliferation and apoptosis.ResultsThe statistically significant expression of herg1 gene was detected in all the gastric cancers and SGC-7901 cells, but not in normal tissues. The HERG K+ channel blocker, E-4031, increased the cell population in G0/G1(P < 0.05) and the number of apoptotic tumor cells(P < 0.05).ConclusionHERG K+ channels were expressed in all gastric carcinomas tested and these channels appear to modulate tumor cell proliferation and apoptosis.