| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 3484123 | Journal of Nanjing Medical University | 2008 | 6 Pages |
ObjectiveTo study the effects and mechanism of homocysteine(Hcy) on Parkinson'sdisease(PD) induced by 6-hydroxydopamine (6-OHDA) in vivo.MethodsForty rats were divided into 4 groups. 6-OHDA or the solvent of 6-OHDA was focally administrated to induce PD, 2 h later Hcy or 0.9% sodium chloride was administrated in the ipsolateral substantial nigra(SN). We used behavioral testing, Immolunohistochemical techniques, biochemistry techniques to detect the injury of SN.ResultsThe rotary turns of PD rats induced by 6-OHDA showed significant increase after treatment with Hcy compared with the controls(P < 0.05). Also the numbers of tyrosine hydroxylase(TH)-stained neurons were decreased, and dendrites were fragmented and truncated. Free radicals were increased and antioxidant enzymes decreased.ConclusionFocal infusion of Hcy into the SN increased the vulnerability of the dopaminergic neurons to 6-OHDA-induced degeneration, it seems that the endangering effect of Hcy is due to exacerbating oxidative stress.
