Selection of DNA aptamer that specific binding human carcinoembryonic antigen in vitro1

ObjectiveTo select the specific aptamer of carcinoembryonic antigen (CEA), one of the most attractive molecule for cancer target therapy and imaging.MethodsSeven rounds in vitro selection were performed against the purified CEA protein. Ligand-mediated target purification and Co-immunoprecipitation were adopted to verify the specific binding of the aptamer to the purified and native protein separately.ResultsThe CEA-specific aptamer which can bind both the purified and native protein with the high specificity was obtained.ConclusionThis is the first time the CEA specific apatmer was produced. The results in this study provides the preliminary evidence for further investigation and application of CEA-aptamer in the future.