| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 3484142 | Journal of Nanjing Medical University | 2007 | 5 Pages |
ObjectiveTo explore the effects of Iptakalim on intracellular free calcium concentration and on the proliferation of cultured rabbit pulmonary arterial smooth muscle cells induced by endothelin-1(ET-1) in vitro.MethodsA cell culture model, [3H]-thymidine([3H]-TdR) incorporation test and confocal microscope were used to observe proliferation and intracellular free calcium concentration([Ca2+]i) of rabbit PASMC induced by ET-1 in vitro.ResultsThe value of [3H]-TdR incorporation in ET-1 group was increased 1.468 times higher than that in control group. Iptakalim at the concentration of 10−7mol/L, 10−6 mol/L, 10−5 mol/L lowered [3H]-TdR incorporation by (19.8 ± 4.6)%, (41.2 ± 9.5)%, (54.7 ± 10.1)%, respectively, compared with the value of the cells treated with ET-1(P < 0.01); The intracellular fluorescence intensity of PASMC in ET-1 group was increased from 73.70 ± 10.12 to 143.84 ± 28.23, significantly higher than that in control group(P < 0.01); whereas with Iptakalim, the fluorescence intensity(FI) was only increased from 74.30 ± 10.20 to 86.03 ± 9.82, significantly lower than that in ET-1 group(P < 0.01).ConclusionIptakalim inhibited proliferation of PASMC and decreased intracellular free calcium concentration of cultured rabbit PASMC induced by ET-1.
