| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 3484163 | Journal of Nanjing Medical University | 2007 | 4 Pages |
ObjectiveReactive oxygen species may be involved in the progression of gastric carcinomas. To clarify whether the pathology of gastric carcinoma are related to oxidative DNA damage, the expression of 8-hydroxy-2′-deoxyguanosine (8-OHdG) was examined in 30 patients with gastric carcinomas.MethodsThe expression of 8-OHdG and apoptosis in the gastric carcinoma were measured using the methods of immunocytochemistry and deoxynucleartididyl transferase-mediated dUTP-biotin nick end labeling (TUNEL), respectively.ResultsOf the 30 cases, 25 (83%) showed stronger immunoreactivity than normal control. The patients with poorly differentiated gastric carcinoma had a larger tumor size and higher labeling indices of TUNEL- and 8-OHdG-positive cells than those with well and moderately differentiated gastric carcinoma.ConclusionOur findings suggest that oxidative DNA damage is increased in association with necroinflammation in chronic gastric injuries and determination of 8-OHdG is useful in assessing high-grade malignancy in gastric carcinomas.
