Renal tubular epithelial cells modulate T-cell responses via ICOS-L and B7-H1

Renal tubular epithelial cells modulate T-cell responses via ICOS-L and B7-H1.BackgroundRenal tubular epithelial cells (TECs) play an active role in renal inflammation. Previous studies have demonstrated the capacity of TECs to modulate T-cell responses both positively and negatively. Recently, new costimulatory molecules [inducible T cell costimulator-L (ICOS-L) and B7-H1] have been described, which appear to be involved in peripheral T-cell activation.MethodsWe characterized expression and regulation of costimulatory molecules on primary human TECs and the TEC line human kidney-2 (HK-2) with reverse transcription-polymerase chain reaction (RT-PCR) and flow cytometry. Immunohistochemistry was performed on human kidney biopsies. The capacity of TECs to modulate T-cell activation was studied in TEC/T-cell cultures.ResultsWe demonstrate that TECs express ICOS-L and B7-H1 in vitro and in vivo. Stimulation with interferon-γ (IFN-γ) resulted in increased expression of B7-H1, whereas ICOS-L expression was marginally increased upon stimulation with CD40L, with no effect of interleukin (IL-1), IL-17, or tumor necrosis factor-α (TNF-α). Furthermore, we show that TECs are able to costimulate T cells that have received signal-1 using αCD3 antibodies, inducing strong IL-10 production, which was partially mediated by ICOS-L. In contrast, B7-H1 appeared to be involved in inhibition of proliferation and cytokine synthesis. In addition, TECs were able to alter the cytokine profile of fully activated T cells, which were incubated with αCD3 and αCD28 antibodies, resulting in low IFN-γ and high IL-10 production. This activity appeared to be independent of ICOS-L and B7-H1.ConclusionInteraction of tubular epithelial cells and kidney infiltrating T cells via ICOS-L and B7-H1 may change the balance of positive and negative signals to the T cells, leading to IL-10 production and limitation of local immune responses.