Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
3910013 | The Breast | 2008 | 8 Pages |
Abstract
Estrogen and iron play critical roles in a female body development and were investigated in the present study in relation to in vitro cell proliferation. Premproâ¢, a hormone replacement therapy drug, and 17β-estradiol (E2) were shown to increase cell proliferations in estrogen receptor positive (ER+) cells independent of progesterone receptor (PR) status. For example, increased cell proliferation was observed in ER+/PR+ human breast cancer MCF-7, its matching non-cancerous human breast epithelial MCF-12A, and ER+/PR+ murine mammary cancer MXT+ cells, but not in ERâ/PRâ MDA-MB-231, its matching non-cancerous MCF-10A, and MXTâ (ERâ/PR+) cells. By mimicking post-menopausal conditions of high estrogen in local breast tissue and increased iron levels due to cessation of menstrual periods, E2 and iron were shown to exert synergistic effects on proliferation of MCF-7 cells and significantly increased Ki67 and proliferating cell nuclear antigen. Western blotting of E2-treated ER+ but not ERâ cells showed that E2 also increased transferrin receptor (TfR). Further studies are needed to assess the mitogenic effects of iron and estrogen in normal post-menopausal breast.
Keywords
Related Topics
Health Sciences
Medicine and Dentistry
Obstetrics, Gynecology and Women's Health
Authors
Jisen Dai, Jinlong Jian, Maarten Bosland, Krystyna Frenkel, Güenther Bernhardt, Xi Huang,