Spermicidal activity of extract from Cestrum parqui

ObjectiveThe leaf extract of Cestrum parqui was examined for its effects on sperm motility in vitro.MethodsWashed sperm were prepared by discontinuous buoyant density gradient centrifugation and incubated with varying concentrations of extract from C. parqui (40–250 μg/mL) at 37°C and 5% CO2. Sperm motility and viability were assessed at different intervals ranging from 5 to 240 min. Morphological changes in human ejaculated spermatozoa after exposure to the extract were evaluated under transmission electron microscope.ResultsA dose- and time-dependent effect of this extract on sperm motility and viability was observed. The maximal spermicidal effect was observed with a 250-μg/mL dose of the extract. Transmission electron microscope revealed a significant damage to sperm membrane in both head and acrosomal membranes, notable swelling and disruption.ConclusionThe present study indicates that this natural extract has potential spermicidal effect in vitro.