Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
3932223 | Fertility and Sterility | 2011 | 4 Pages |
Abstract
The successful identification of spermatozoa at microdissection testicular sperm extraction is critical and can depend on the judgment of experienced reproductive clinicians. Therefore, we characterized human spermatogenic cells based on stage-specific mitochondrial location and morphologic change by using a vital mitochondrion-specific fluorescent probe, Mitotracker, and stage-specific antibodies to make a useful microscopic diagram.
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Authors
Hidenobu Okuda, Akira Tsujimura, Keisuke Yamamoto, Shinichiro Fukuhara, Jiro Nakayama, Hiroshi Kiuchi, Tetsuya Takao, Yasushi Miyagawa, Norio Nonomura, Akihiko Okuyama,