Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
3932836 | Fertility and Sterility | 2010 | 6 Pages |
ObjectiveTo examine sperm motility, total antioxidant level (TAC), DNA fragmentation, and medical history in infertile men with high seminal high reactive oxygen species (ROS).DesignProspective study.Setting(s)Tertiary care hospital.Patient(s)Infertile men (n = 101).Intervention(s)Group I (n = 57) included men with seminal ROS (<250 relative light units/sec/×106 sperm) while group II (n = 44) included men with seminal ROS levels (≥250 relative light units/sec/×106 sperm).Main Outcome Measure(s)Seminal ROS, TAC, sperm DNA fragmentation, ROS/TAC score were measured.Result(s)Group II had a high incidence of sperm DNA fragmentation than group I. The odds ratio of 1.25 for elevated ROS levels corresponded to >10% greater DNA fragmentation in our patients (95% confidence interval 1.01–1.53). Group II showed poor motility, a higher incidence of leukocytospermia, and higher ROS-TAC scores compared with group I. ROS was negatively correlated with sperm curvilinear velocity (r = −.24), linearity (r = −.24), and sperm motility (r = −.31). Sperm motility was correlated with %TUNEL+ve sperm (r = −.39).Conclusion(s)An increase in seminal ROS levels by 25% was associated with a 10% increase in sperm DNA fragmentation. Sperm motility was affected by seminal ROS and sperm DNA fragmentation.