Intrafollicular antimüllerian hormone levels predict follicle responsiveness to follicle-stimulating hormone (FSH) in normoandrogenic ovulatory women undergoing gonadotropin releasing-hormone analog/recombinant human FSH therapy for in vitro fertilization

ObjectiveTo investigate the relationship between antimüllerian hormone (AMH) and steroidogenesis in follicles of normoandrogenic ovulatory women undergoing follicle-stimulating hormone (FSH) therapy for in vitro fertilization/embryo transfer (IVF-ET).DesignProspective cohort.SettingInstitutional/private practice.Patient(s)26 normoandrogenic ovulatory women. All women received gonadotropin-releasing hormone (GnRH) analog and ovarian stimulation for IVF-ET.Intervention(s)Follicle fluid was aspirated at oocyte retrieval from the first follicle of each ovary.Main Outcome Measure(s)Follicle fluid was assayed for AMH, estradiol (E2), progesterone, androstenedione, testosterone, dihydrotestosterone, insulin, and FSH.Result(s)Intrafollicular AMH levels positively and negatively correlated with E2 and FSH concentrations in follicles, respectively, causing a positive relationship between follicle fluid AMH levels and E2/FSH ratios as a measure of follicle sensitivity to FSH. A positive relationship also existed in follicles between AMH levels and E2/androgen ratios as a marker of aromatase activity.Conclusion(s)The AMH levels in follicles of IVF patients positively correlate with follicle sensitivity to FSH.