Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
3938511 | Fertility and Sterility | 2010 | 5 Pages |
Abstract
Deoxyribonucleic acid fragmentation and oxidative DNA damage were simultaneously determined in the same sperm cell, incubating with an 8-oxoguanine DNA probe on human spermatozoa processed by the sperm chromatin dispersion test. The assay was validated by incubation with agents that induce DNA fragmentation with or without oxidative base damage. In all samples examined, increased levels of 8-oxoguanine were present only in those spermatozoa with fragmented DNA, suggesting a link between both DNA damage types.
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Authors
Rebeca Santiso, María Tamayo, Jaime Gosálvez, Marcos Meseguer, Nicolás Garrido, José Luis Fernández,