Use of metaphase I oocytes matured in vitro is associated with embryo multinucleation

Embryo multinucleation rate increases when in vitro-matured (2-5 hours incubation) MI (MI-II) oocytes are used instead of in vivo-matured oocytes in ICSI. Furthermore, all other ICSI outcome parameters are also compromised. The use of donated gametes does not modify these results.