Cryopreservation has no effect on meiotic recombination and synapsis in testicular tissues

The effects of cryopreservation on meiotic progression, synapsis, recombination, and structure of synaptonemal complexes (SCs) in testicular tissues were evaluated by comparing the above-mentioned parameters in frozen and fresh testicular tissues from the same men. No differences in meiotic progression, the mean number of MLH1 foci per cell, the mean number of autosomal SCs with different numbers of MLH1 foci, or the fidelity of the synapsis were observed between fresh and frozen testicular tissues.