Dienogest inhibits BrdU uptake with G0/G1 arrest in cultured endometriotic stromal cells

ObjectiveTo investigate the effect of dienogest on the proliferation of endometriotic stromal cells.DesignComparative and laboratory study.SettingUniversity of Tokyo Hospital.Patient(s)Endometriotic stromal cells were isolated and cultured from ovarian endometriomas of patients undergoing surgery.Intervention(s)Dienogest was added to the cultured endometriotic stromal cells.Main Outcome Measure(s)5-Bromo-2′-deoxyuridine (BrdU) incorporation into DNA of the endometriotic stromal cells was measured by ELISA. Cell cycle analysis of the cultured endometriotic stromal cells was performed by flow cytometry.Result(s)Dienogest at concentration of 10-7 M and 10-6 M significantly inhibited BrdU incorporation into DNA at 24 and 48 hours. Dienogest significantly increased the cells in G0/G1 phase and reduced the cells in S phase and G2/M phase in 24 and 48 hours.Conclusion(s)The present study indicates that dienogest can inhibit the proliferation of the endometriotic stromal cells with G0/G1 arrest, suggesting a possible direct effect of dienogest in the treatment of endometriosis.