| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 3941790 | Fertility and Sterility | 2008 | 6 Pages |
ObjectiveTo quantify p27 messenger RNA (mRNA) levels in human arrested and normally developing embryos and nonfertilized oocytes to determine whether the p27 protein abundance, reported in cleavage-stage embryos exhibiting developmental arrest, is regulated at the mRNA expression level.DesignReal-time reverse transcription quantitative polymerase chain reaction was used to quantify the expression of p27 in three samples: arrested embryos (group A, n = 29), normally developing embryos (group D, n = 34), and nonfertilized oocytes (group O, n = 20).SettingResearch laboratory working closely with a clinical IVF practice.Patient(s)Oocytes and embryos were obtained from patients undergoing assisted fertilization.Intervention(s)None.Main Outcome Measure(s)Quantification of mRNA transcripts.Result(s)The amount of p27 mRNA was statistically significantly higher in group A (mean ± SEM, 86,143 ± 42,496 relative units [RU]) compared with groups D (10,680 ± 3,850 RU) and O (3,555 ± 1,458 RU). Furthermore, in a group of 13 two- to four-cell arrested embryos, high levels of p27 mRNA (51,481 ± 31,120 RU) were found in comparison with the nonfertilized oocyte group (3,555 ± 1,458 RU).Conclusion(s)Cleavage-stage human embryos exhibiting developmental arrest show increased p27 mRNA expression. This probably is due to increased transcriptional activity.
