p16 and MIB1 improve the sensitivity and specificity of the diagnosis of high grade squamous intraepithelial lesions: Methodological issues in a report of 447 biopsies with consensus diagnosis and HPV HCII testing

Several important methodological issues have been studied. Overall, p16 and MIB1 are promising markers to help pathologists differentiate HG lesions from all else. The staining of the endocervix and the ectocervix do not always agree, and the ectocervix more often stains positive with the presence of HGSIL. Each marker is helpful and both are helpful together. In conclusion, both markers are useful for the confirmation of HG lesions.