Cloning and Character Analysis of A Novel Testis-specific Gene, TSF22, in Mice

ObjectiveTo identify genes that involved in spermatogenesis.MethodsIn order to screen the testis-specific genes, testes cDNA samples from BALB/c mice of different postnatal days (days 4, 9, 18, 35, 54 and 6 months) were performed with mouse whole genome Affymetrix chip. The characteristics of the selected gene were analyzed by various bioinformatic tools. The expression profile of the selected gene was identified by RT-PCR.ResultsBy analyzing the hybridization signals, a gene with a differential expression in the developmental stages of testis was identified. This gene was designated as TSF22.The full length cDNA of 1 597 bp contained an open reading frame of 570 bp which encoded a putative protein of 190 amino acids and a molecular weight of 22.106 kD. RT-PCR analysis revealed that TSF22 mRNA was exclusively expressed in mice testis.ConclusionsTSF22, functions as a testis-specific transcription factor, may play important roles during spermatogenesis.