Root Extract of Polygonum Hydropiper Alters the Expression of Rat Uterine Protein Profile in Presence and Absence of Ovary in-situ during Periimplantation Period: An Evidence on SDS-PAGE

ObjectiveTo study its effects on reproductive performance in albino rats.MethodsThe chromatographic fraction (CF) of crude methanolic extract of the herb has been subcutaneously administered to female albino rats. Experiments were carried out in adult cyclic females and oavriectomised (OVX) females during early gestation period. Uterine horns were collected following the respective treatment regimen to study the protein profile in 15% gel SDS-PAGE.ResultsThe CF induced changes in the expression of protein in rat uterus. New proteins have been expressed in uterus of adult cyclic females having ovary in-situ. The OVX females treated with CF showed altered uterine protein profile compared with that of OVX control and OVX estradiol-17β (E2) treated rats. The CF exerted its effect on expression of uterine protein during early gestation period in rats. While uterine proteins of CF treated females were similar to that of controls during preimplantation period; many of the proteins on day 6 of gestation have been found either missing or expressed in lesser intensity.ConclusionThe root of Polygonum hydropiper contains potential compound(s) which can alter the reproductive performance of female rats modulating uterine protein expression.