| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 4032212 | Progress in Retinal and Eye Research | 2006 | 28 Pages |
Abstract
Most of the recently discovered corneal anti-angiogenic factors belong to extracellular matrix proteins that aquire angio-inhibitory activity only after proper proteolytic processing. Our recent findings showed that the secretion of endostatin (derived from basement membrane collagen XVIII) and restin (from collagen XV) by HLCECs were enhanced when HLCECs were cultivated on amniotic membrane (AM). This adds to the advantage of transplanting ex vivo expanded HLCECs cultivated on AM in that the anti-angiogenic activity of the epithelial cells is augmented in a physiological way. Furthermore, proteomic profiling of HLCECs and human conjunctival epithelial cells (HCECs) identified a 14-3-3 protein (stratifin) preferentially expressed by HLCECs. In addition to functioning as a cell cycle controller, keratinocyte-derived stratifin induces MMPs which are involved in the generation of restin (by MMP-1) and endostatin (by MMP-3). These findings highlight the significance of delicate epithelial-matrix interactions in the maintenance of corneal avascularity.
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Authors
David Hui-Kang Ma, Jan-Kan Chen, Fen Zhang, Kuei-Ying Lin, Jeng-Yuan Yao, Jau-Song Yu,