Establishing primary cultures of vascular smooth muscle cells from the spiral modiolar artery

We obtained purified VSMCs using this method. All cell cultures expressed smooth muscle markers (α-SM actin, and myosin) and were negative for vWF. This article provides a simple method to obtain VSMCs for in vitro studies of physiology and pathophysiology in the circulation disturbances of the inner ear. In addition, VSMCs are regarded to be an excellent model to evaluate drugs in vitro.