Quantification de l'apoptose in vivo : comparaison de méthodes de détection sur coupes histologiques de tumeurs xénogreffées issus de la lignée cellulaire humaine d'adénocarcinome colique HT-29

Immunohistochemistry for activated caspase-3 and cleaved cytkeratin-18 appear to be an easy, sensitive, and reliable method for detecting and quantifying apoptosis in this model. There are therefore recommended for the detection and quantification of apoptosis in tissue sections. Other markers as cleaved PARP-1 and activated caspase-7 can be an interessant solution: advantages and inconvenients for each methods are exposed.