Mannitol has a protective effect on testicular torsion: An experimental rat model

The seminiferous tubule structure in testicular torsion without mannitol treatment was significantly disturbed, whereas the structural disruption was considerably less in the mannitol group. Mannitol treatment also decreased reactive oxygen radical levels significantly and was able to decrease apoptosis. These results were consistent with other organ model studies that evaluated the protective effects of mannitol treatment in I/R injury. Mannitol infusion had a protective effect against I/R injury in testicular torsion in rats. This experimental study may guide clinicians to evaluate the effectiveness of mannitol in human testicular torsion.Table. Histopathological evaluation of study groups.Apoptotic indexPCNA indexMTBSMSTDGroup A3.88 ± 0.83b36.47 ± 1.36b9.23 ± 0.17b273.25 ± 5.14bGroup B25.88 ± 2.91a,b22.22 ± 2.34a,b4.57 ± 0.22a,b209 ± 6.11a,bGroup C25.52 ± 2.87a,b22.51 ± 2.99a,b4.76 ± 0.14a,b210.63 ± 5.09a,bGroup D15.25 ± 1.48a29.28 ± 1.66a6.72 ± 0.25a229.63 ± 6.18aMSTD = mean seminiferous tubular diameter; MTBS = mean testicular biopsy score; PCNA = proliferating cell nuclear antigen; I/R = ischemia-reperfusion.Group A was a sham operation group, Group B (I/R) had 3 h ischemia and 2 h reperfusion, Group C (I/R + Saline) had 3 h ischemia and 2 h reperfusion and saline bolus treatment, Group D (I/R + Mannitol) had 3 h ischemia and 2 h reperfusion and mannitol bolus treatment.ap < 0.01 compared with group A.bp < 0.01 compared with group D.